June 2002 overview this microbiology laboratory manual is designed especially for the nonmajors, health science. Laboratory biosafety guidance related to the novel coronavirus 2019ncov. Introduction to the microbiology of food processing united states department of agriculture 5 unfortunately, microorganisms also can be detrimental. Microbiology laboratory safety rules and procedure learn. The safe handling of microorganisms in the teaching laboratory is a top priority. This procedure applies to all employees and students at tru. Food safety divided into causitive categories called hazards biological hazards bacteria, molds, natural occurring toxins chemical hazards chemicals like petroleum, herbicides, pesticides, heavy metals. Bunsen burner bunsen burner is a common tool used in science lab fig. Microbiology laboratory safety rules and procedures. Those rules or procedures listed in your laboratory manual that appear to be in conflict with those given below should be resolved by asking your course instructor. These procedures will assist laboratories in attaining the competence required, will enhance the quality of data reported and ensure the safety of laboratory workers. Nine safe practices for the microbiology laboratory. Pharmaceuticals, nutraceuticals, cosmetics, personal care products, consumer products, disinfectants, antimicrobial products and device development, food safety and quality, biofouling. These include using model risk assessments for which it is necessary to refer to appropriate publications such as cleapss laboratory handbook 2006, section 15.
Laboratory safety manual the laboratory safety manual is intended to address universal safety measures for achieving a safe and healthy working environment. For courses in microbiology lab and nursing and allied health microbiology lab. Standard safety practices in the microbiology laboratory. People must be advised of potential hazards before entering the work area. Learn 1 microbiology lab safety with free interactive flashcards. Microbiology laboratory safety and rules biology ii mrs. Msds for all dangerous chemicals must be kept in the laboratory.
Bsl1 lab is used to perform research mostly on noninfectious microbes using standard equipment and routine lab safety procedures. Laboratory doors should be kept closed and access to laboratory areas must be limited and controlled. The lab safety plan will provide more detailed safety information for the hazards present in the lab. It describes good laboratory practices that must be understood and observed by all individuals involved in the laboratory. Safety procedures and precautions in the microbiology laboratory all safety procedures and precautions followed in the microbiology laboratory are designed to. Oversees laboratory safety within departmental laboratories ensures laboratories complete and update annual inventories of hazardous chemicals as required by the universitys hazard communication program if authorized by the dean, may appoint a laboratory safety coordinator. Evacuate the area, use stairs and close doors behind you. Ppt introduction to microbiology and laboratory safety. The basic objectives of a microbiological research laboratory, i protection of the experimenter and staff, ii protection of the surrounding community, and iii maintenance of experimental validity, are defined. In addition to microorganisms, there are some chemicals used in this laboratory that are potentially harmful. Wear proper attire for the microbiology lab at all times lab coat that comes down to. In 1970, it became the microbiology institute of hubei province when the hubei commission of science and. Wash your hands with disinfectant soap when you arrive at the lab and again before you leave.
Move away from the building exterior and assemble with. Biosafety in microbiological laboratories flashcards quizlet. The following safety guidelines listed below apply to all microbiology laboratories, regardless of biosafety level. To ensure safer downloads, we have uploaded this pdf ebook file to our online file repository. Bsl4 level is mandatory for research on viruses and bacteria that cause severe to fatal disease in humans, and for which no vaccines or treatments are available. Quality assurance for microbiology in feed analysis. Report all incidents spills, accidents, injuries, thefts to the health and safety representative of the laboratory and safety officer a311. Pdf on oct 10, 2008, abu nayeem and others published an assignment of microbiology laboratory use of microbiology lab, chemical and equipment find, read and cite all the research you need on. Large fires activate the nearest fire alarm pull station and alert others. Safety in a microbiology laboratory is important in the prevention of infection as microbiology laboratory cultures, manipulates, and uses virulent andor potentially pathogenic microorganisms. Start studying biosafety in microbiological laboratories. Jan 14, 2009 part 4 of 5 part series on safety in the microbiology laboratory. Standard precautions are based on the principle that all blood, body fluids, secretions, nonintact skin, mucous membranes, and excretions except sweat may contain transmissible infectious agents.
With a portfolio of over 65,000 products from suppliers, fisher safety has access to the most respected brands of safety and ppe products, allowing us to serve as your single source for thousands of safety products. Chemical spill kits are available at the emergency shower. Microbiology laboratory safety and basic procedures safety in a microbiology laboratory is important in the prevention of infection that might be caused by the microorganisms being studied. The microbiology laboratories are certified as pc2 physical containment level. The laboratory safety manual is intended to address universal safety measures for achieving a safe and healthy working environment. Biosafety in microbiological and biomedical laboratories bmbl quickly became the cornerstone of biosafety practice and policy in the united states upon first publication in 1984. Unc greensboro environmental health and safety 1 laboratory safety sign program 1. It is through their absolute commitment to quality, technical accuracy, and dedication to the professional.
In addition to safety risks associated with any clinical lab. In addition, students must practice safe laboratory techniques at all times. You will be doing many laboratory activities which require the use of potentially hazardous bacteria and chemicals. Learn vocabulary, terms, and more with flashcards, games, and other study tools. It develops and explains the concept of primary and secondary barriers for the containment of microorganisms. The mlg contains methods for the sample preparation, isolation and identification of the major foodborne pathogenic microorganisms and their toxins, meat tissue species identification, and the. A laboratory manual pdf using our direct download link mentioned at the end of this article. The microbiology laboratory guidebook mlg contains test methods used by the fsis field service laboratories to support fsis regulatory activities. Some of these rules and procedures are listed in the beginning material of your laboratory manual. Oct 15, 2010 interview with the devil the secret to freedom and success napoleon hill duration. The third edition of the who laboratory biosafety manual is a helpful reference and guide to nations that accept the challenge to develop and establish national codes. For advanced ecommerce capabilities fisher safety leads the industry in providing advanced ecommerce solutions for. A free powerpoint ppt presentation displayed as a flash slide show on id. Microbiology a laboratory manual 11th edition 2016 pdf.
Alright, now in this part of the article, you will be able to access the free pdf download of microbiology. To ensure the safety of people in the microbiology laboratories at thompson rivers university tru. Introduction to microbiology and laboratory safety biosafety for micr 2909 lecture 2, 2001 bscmolbiol lect 2. Historically, the information in this publication has been advisory is nature even though legislation and regulation, in some circumstances, have overtaken it and made compliance with the guidance provided mandatory. Microbiology module laboratory safety and standards precautions microbiology 70 notes 6. Introduction to the microbiology of food processing. Our microbiology labs offer a wide array of analytical testing services to support the following industries or fields. Safety in the microbiology laboratory biology libretexts.
The 3m molecular detection system now offer two new assays to the testing portfolio. Laboratory safety manual university of texas at austin. Safety procedures and precautions in the microbiology. Safety in microbiology laboratory pdf safety in the microbiology laboratory.
The third edition of the who laboratory biosafety manual is a helpful reference and guide to nations that accept the challenge to. Laboratory personnel must receive training regarding the laboratory standard, the chp, and other laboratory safety practices, including exposure detection, physical and health hazards associated with chemicals, and protective measures. I reduce or eliminate exposure of lab workers, other persons, and the outside environment to potentially hazardous microorganisms. The legs are a vulnerable area if uncovered, so it is inappropriate to wear. Microbiology lab safety thompson rivers university.
A laboratory manual serves as a general laboratory manual for undergraduate and graduate students in food microbiology, as well as a training manual in analytical food microbiology. Pdf an assignment of microbiology laboratory use of. Lab coats, closedtoe shoes, and gloves prevent biohazardous materials from contact with the skin, including areas where there might be cuts, abrasions, or dermatitis. However, many types of microorganisms are potentially pathogenic. The equipment is electrically powered and uses ammonia as the refrigerant. Limiting access to laboratory sometimes, people who do not work in the laboratory attempt to enter the laboratory to look for test results they desire. The use of a positivepressure personnel suit is mandatory as well as many additional safety measures of the labs. Introduction the uncg laboratory safety sign program is designed to alert laboratory personnel, staff, students, visitors, and emergency responders to the specific hazards located in individual laboratories on campus. The microbiology laboratory guidebook mlg contains test methods used by the. List of equipment apparatus used in microbiology laboratory.
The three main elements of safe containment of microorganisms are 1. Familiarize yourself with the operation of the fire. Upon completion of the lecture, accompanying video, required readings and clinical rotations, the student will be able to. The three main elements of safe containment of microorganisms are 1 good laboratory practices and technique, 2 safety equipment, and 3 facility design. I reduce or eliminate exposure of lab workers, other persons. At every step, 3m provides solutions that help mitigate risk, improve operational efficiencies and impact the bottom line. Access to the laboratory is limited or restricted at the discretion of. Will serve as the lsc in the absence of the coordinator. Microbiology laboratory safety rules and procedures crcooper01. Microbiology laboratory safety rules and procedures each student enrolled in a microbiology laboratory at youngstown state university must follow specific safety rules and procedures.
Microbes in action activities are not pathogenic for humans. The guidelines are straightforward and largely common. The following standard and special practices, safety equipment and facilities apply to agents assigned to biosafety level 1. Mount sinai hospital general laboratory safety manual laboratory centre for disease control centers for disease control and national institutes of health laboratory safety csmls guidelines clinical microbiology procedures handbook 2nd edition the laboratory safety manual is intended to address universal safety measures for achieving a safe and. Bsl2 work is performed with bacteria and viruses that cause only mild disease to humans, or are difficult to contract via aerosol in a lab setting. Report all incidents spills, accidents, injuries, thefts to the health and safety representative of. Microbiology lab safety printed copies of this document are uncontrolled.
All safety procedures and precautions followed in the microbiology laboratory are designed to. In 1961, it became the south china institute of microbiology, and in 1962 was renamed to wuhan microbiology institute. Safety information safety guidelines microbiology online. Sep 25, 2019 the laboratory has special engineering and design features. The institute was founded in 1956 as the wuhan microbiology laboratory under the chinese academy of sciences cas. Safety procedures and precautions in the microbiology laboratory. Choose from 500 different sets of 1 microbiology lab safety flashcards on quizlet. Safety in the microbiology laboratory although the organisms used in all science in the real world. Biosafety in microbiological and biomedical laboratories. The laboratory shall have means of securing specifically regulated materials such as dea.
Safety data sheet msds when working with a chemical for the first time. Safety at work act to comply with any safety instructions given by their employers. Objectives students successfully completing this laboratory exercise should be able to. Although the organisms used in all science in the real world.
Laboratory safety guidance occupational safety and. Microbes in action activities are not pathogenic for humans, students should be taught to handle them as they would if they were unsure about the dangers. A microbiology laboratory is a unique environment that requires special practices and containment facilities in order to properly protect persons working with microorganisms. These include using model risk assessments for which it is necessary to refer to appropriate publications such as cleapps laboratory handbook 2006, section 15. Pineapple research station k erala agricultural university, vazhakulam686 670, muvattupuzha. The absolute cabinet system terminates with a doubledoored autoclave, and. Laboratory manual and workbook in microbiology applications to patient care 7th edition josephine a. Diseasecausing microorganisms are called pathogens. Easy to adapt for almost any microbiology lab course, this versatile, comprehensive, and clearly written manual is competitively priced and can be paired with any undergraduate microbiology text.